Histological techniques allow us to represent physiological or pathophysiological conditions in two dimensions - usually by light microscopic investigation of thin tissue slices.
To go beyond this imposed limitation of conventional histology, micro computed tomography offers an extension into three dimensional tissue characterization.
The authors were able to link conventional histological techniques, including scanning electron microscopy, to synchrotron radiation based micro computed tomography (SR-ВµCT). SR-ВµCT allowed characterizing the morphology of an articular cartilage tissue sample in three dimensions and was able to resolve individual cells, with the possibility to quantify cell number and orientation.
Journal of the Royal Society Interface
Journal of the Royal Society Interface is the Society's cross-disciplinary publication promoting research at the interface between the physical and life sciences. It offers rapidity, visibility and high-quality peer review and is ranked fifth in JCR's multidisciplinary category. The journal also incorporates Interface Focus , a peer-reviewed, themed supplement, each issue of which concentrates on a specific cross-disciplinary subject.
rsif.royalsocietypublishing
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